Friday, December 18, 2009

Annealing Temp Primer Calculator Melting Temperature And Annealing Temp Of PCR?

Melting temperature and Annealing temp of PCR? - annealing temp primer calculator

Hello ... I know that is the melting temperature of primers in the .. 50% of lost duplexes are unique, but does so with the annealing temperature? Why must we always get a lower annealing temperature above the melting temperature? Please help me with this

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